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  • Recombinant Human SCF, GMP Grade

Recombinant Human SCF, GMP Grade

Synonyms:

Kit ligand, Stem cell factor, SCF, c-Kit ligand, Mast cell growth factor, MGF

Species:

Human

Cat. No.:

CRS200B-GMP pdf (datasheet)

Quantity/Size:

100 μg

Price:

$2,355.00 BUY

Description:

Stem cell factor (SCF), is a cytokine made by fibroblasts and endothelial cells. SCF binds to the receptor c-Kit/CD117 and plays a critical role in the maintenance, survival, and differentiation of hematopoietic stem cells. While human SCF shows no activity on murine cells, murine and rat SCF are active on human cells.
This product is produced from E. coli and is manufactured in a facility that does not use or process beta-lactam containing materials. No animal- or human-derived materials were used during manufacturing or as ingredients. This product is manufactured, tested, and released in an ISO 9001:2015 certified facility and follows cGMP practices. USP chapter <1043> for ancillary materials considered in the manufacture of this product.

Entrez Symbol:

KITLG

Uniprot Name:

Kit ligand

Uniprot ID:

P21583

Data PDF:

CRS200-GMP

Source:

E. coli

Molecular Weight:

18.6 kDa predicted (165 aa), monomer

Formulation:

Lyophilized (1 mg/ml) sterile-filtered 10 mM sodium phosphate, 50 mM NaCl, pH 7.5

Purity:

> 97% by reducing and non-reducing SDS-PAGE

Biological Activity:

ED50 < 15 ng/ml, determined by dose-dependent proliferation of human TF-1 cells.

Specific Activity:

~1.0 x 10e3 IU/µg, calibrated against recombinant human stem cell factor WHO International Standard (NIBSC code 91/682).

Endotoxin Level:

< 0.05 EU/µg using USP <85>/ EP 2.6.14

Amino Acid Sequence:

MEGICRNRVT NNVKDVTKLV ANLPKDYMIT LKYVPGMDVL PSHCWISEMV VQLSDSLTDL LDKFSNISEG LSNYSIIDKL VNIVDDLVEC VKENSSKDLK KSFKSPEPRL FTPEEFFRIF NRSIDAFKDF VVASETSDCV VSSTLSPEKD SRVSVTKPFM LPPVA

Storage/Stability:

Lyophilized vial is shipped at ambient temperature. Immediately upon receipt, store unopened vial at -20°C (manual defrost freezer) or -80°C for up to 36 months. Upon reconstitution, prepare working aliquots and store at -20°C (manual defrost freezer) or -80°C. It is recommended that a carrier protein such as 0.1% HSA or BSA is added for long term storage. For short term storage reconstituted material is stable for 4-6 weeks when stored at 2-8°C. Avoid repeated freeze-thaw cycles.

Application notes:

For ex vivo use only. Not for injection or diagnostic procedures. The safety and efficacy of this product in diagnostic or other clinical uses has not been established.