Recombinant human PDGFR-beta contains the catalytic domain, amino acids 558-1106, with a 39 aa N-terminal addition including a 6x His tag.

Amino Acid Sequence

MSYYHHHHHH DYDIPTTENL YFQGAMLVPR GSPWDPEFQK KPRYEIRWKV IESVSSDGHE YIYVDPMQLP YDSTWELPRD QLVLGRTLGS GAFGQVVEAT AHGLSHSQAT MKVAVKMLKS TARSSEKQAL MSELKIMSHL GPHLNVVNLL GACTKGGPIY IITEYCRYGD LVDYLHRNKH TFLQHHSDKR RPPSAELYSN ALPVGLPLPS HVSLTGESDG GYMDMSKDES VDYVPMLDMK GDVKYADIES SNYMAPYDNY VPSAPERTCR ATLINESPVL SYMDLVGFSY QVANGMEFLA SKNCVHRDLA ARNVLICEGK LVKICDFGLA RDIMRDSNYI SKGSTFLPLK WMAPESIFNS LYTTLSDVWS FGILLWEIFT LGGTPYPELP MNEQFYNAIK RGYRMAQPAH ASDEIYEIMQ KCWEEKFEIR PPFSQLVLLL ERLLGEGYKK KYQQVDEEFL RSDHPAILRS QARLPGFHGL RSPLDTSSVL YTAVQPNEGD NDYIIPLPDP KPEVADEGPL EGSPSLASST LNEVNTSSTI SCDSPLEPQD EPEPEPQLEL QVEPEPELEQ LPDSGCPAPR AEAEDSFL

Background: PDGFR-beta is a cell surface tyrosine kinase receptor for members of the platelet-derived growth factor family. These growth factors are mitogens for cells of mesenchymal origin. The PDGF isoforms AA, BB, AB, CC, and DD are dimeric molecules. They bind two receptor molecules simultaneously and dimerize them upon binding. PDGFR-alpha binds the A, B, and C chains of PDGF with high affinity, whereas PDGFR-beta binds the B and D chain with high affinity.