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Recombinant Human Cardiotrophin-1

Molecule:

Cardiotrophin-1

Synonyms:

Cardiotrophin-1, CT-1

Species:

Human

Cat. No.:

CRC700A pdf (datasheet)

Quantity/Size:

10 μg

Price:

$425.00 BUY

Description:

Cardiotrophin-1 (CT-1) is a member of the Leukemia Inhibitory (LIF)/ Ciliary Neurotrophic Factor (CNTF)/ Oncostatin M (OSM)/ Interleukin-6 (IL6)/ Interleukin-11 (IL11) family of cytokines. It affects cells through interaction with the gp130 (ILST) receptor subunit and stimulates the Janus kinase/signal transducers and activators of transcription (JAK/STAT) pathway to transduce hypertrophic and cytoprotective signals in cardiac myocytes. CT 1 has also a neurotrophic function. CTF1 deficiency causes increased motoneuron cell death in spinal cord and brainstem nuclei of mice during a period between embryonic day 14 and the first postnatal week. Moreover, CT-1 is a hepatocyte survival factor that efficiently reduces hepatocellular damage in animal models of acute liver injury. Cardiotrophin 1 expression is augmented after hypoxic stimulation and it can protect cardiac cells when added either prior to simulated ischaemia or at the time of reoxygenation following simulated ischaemia. Cardiotrophin 1 can induce expression of the protective heat shock proteins (hsps) in cardiac cells.

Entrez Symbol:

CTF1

Uniprot Name:

Cardiotrophin-1

Uniprot ID:

Q16619

Data PDF:

CRC700

Source:

E. coli

Molecular Weight:

21.2 kDa (201 aa) monomer

Tag:

None

Formulation:

Lyophilized from a sterile filtered aqueous solution containing 30% acetonitrile, 0.1% TFA

Purity:

> 95% by SDS-PAGE and RP-HPLC

Biological Activity:

ED50 <1.0 ng/ml, determined by a cell proliferation assay using human TF-1 cells.

Specific Activity:

> 1.0 x 10e6 IU/mg

Amino Acid Sequence:

MSRREGSLED PQTDSSVSLL PHLEAKIRQT HSLAHLLTKY AEQLLQEYVQ LQGDPFGLPS FSPPRLPVAG LSAPAPSHAG LPVHERLRLD AAALAALPPL LDAVCRRQAE LNPRAPRLLR RLEDAARQAR ALGAAVEALL AALGAANRGP RAEPPAATAS AASATGVFPA KVLGLRVCGL YREWLSRTEG DLGQLLPGGS A

Reconstitution:

Centrifuge the vial prior to opening. Reconstitute in sterile 4 mM HCl not less than 0.10 mg/mL. The solution can be further diluted to other aqueous buffers.

Storage/Stability:

Store as supplied at -20°C to -80°C for up to 1 year. Upon reconstitution store at 2-8°C for up to one week, or long term in aliquots -80°C. Avoid repeated freeze-thaw cycles.