Mouse Anti-Human PDGF-B (Clone 5F66) mAb

Molecule:

PDGFB

Synonyms:

Platelet-derived growth factor subunit B, PDGF subunit B, PDGF-2, Platelet-derived growth factor B chain, Proto-oncogene c-Sis

Species:

Human

Cat. No.:

CMP107 pdf (datasheet)

Quantity/Size:

100 μg

Price:

$800.00 BUY

Description:

Platelet-Derived Growth Factor beta (PDFGB) is a member of the Platelet-Derived Growth Factor family. The four members of this family are mitogenic factors for cells of mesenchymal origin and are characterized by a motif of eight cysteines. PDGFB can exist either as a homodimer (PDGF-BB) or as a heterodimer (PDGF-AB) with PlateletDerived Growth Factor alpha (PDGFA), where the dimers are connected by disulfide bonds. Mutations in the PDGFB gene are associated with meningioma. Reciprocal translocations between chromosomes 22 and 7, at sites where this gene and that for COL1A1 are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans resulting from unregulated expression of growth factor. Two alternatively spliced transcript variants encoding different isoforms have been identified for this gene.

Uniprot Name:

Platelet-derived growth factor subunit B

Uniprot ID:

P01127

Data PDF:

CMP107

Specificity:

Recognizes Human PDGF-BB

Host Species:

Mouse

Immunogen:

Recombinant Human PDGF-BB

Isotype:

IgG1

Clone:

5F66

Formulation:

Lyophilized from sterile filtered PBS

Purification:

Protein G affinity chromatography

Reconstitution:

Centrifuge vial briefly prior to opening. Add 500 µl sterile PBS to the vial for a concentration of 200 µg/ml. Gently mix, allow a few minutes to solubilize completely. Do not vortex.

Application notes:

Western Blot: suggested dilution 1:1000
IHC-P: suggested dilution 1:50-1:200

Storage/Stability:

Lyophilized antibody is stable for at least 1 year at -20°C to -80°C. Reconstituted antibody may be prepared as aliquots and stored at -20°C to -80°C for at least six months. Avoid repeated freeze-thaw cycles.