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Mouse Anti-Histidine Tag (Clone HIS.H8) mAb

Molecule:

His

Synonyms:

Polyhistidine tag, 6xHis-tag, His6 tag

Cat. No.:

CSI20563A pdf (datasheet)

Quantity/Size:

50 μg

Price:

$210.00 BUY

Concentration:

1.0 mg/ml

Description:

The mouse monoclonal antibody recognizes the epitope of 6x His-tags encoded by many commercially available vectors, regardless of the tag's location in the fusion protein sequences, Reacts with N-terminal, C-terminal or internal 6x His-tags.

Data PDF:

CSI20563

Specificity:

Reacts with N-terminal, C-terminal or internal 6x His-tags.

Host Species:

Mouse

Immunogen:

HHHHHH (6x His) synthetic peptide conjugated to KLH

Isotype:

IgG2b

Clone:

HIS.H8

Conjugate:

Unconjugated

Formulation:

Liquid in 10 mM PBS, pH 7.2, with 0.05% NaN3.

Purification:

Protein A affinity chromatography from mouse ascites fluid

Application:

DB , ELISPOT, IF, IP , WB

Application notes:

Western Blot (with ECL) : 1:1,000-3,000 dilution (1 hour at room temperature).
Immunoprecipitation: 1:50-200 dilution
Immunofluorescence: 1:500-2,000 dilution

The optimal concentration should be determined by the user for each specific application.

Storage/Stability:

Store at -20°C to -80°C for up to 1 year. Centrifuge after first thaw to maximize product recovery. Aliquot and store at 2-8°C for 1-2 weeks, or long-term at -20 to -80°C. Avoid repeated freeze-thaw cycles.