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Research Grade Anti-SARS-CoV-2 Spike Protein/RBD Broad-Neutralizing Antibody (002-S21F2)

Synonyms:

Spike glycoprotein, Spike receptor binding domain, Spike-RB protein, S2E12

Species:

SARS-CoV-2

Cat. No.:

DVV00332B pdf (datasheet)

Quantity/Size:

1.0 mg

Price:

$1,715.00 BUY

Concentration:

1.0 mg/ml, lot specific

Description:

Recombinant Human anti-SARS-CoV-2 Spike-RBD (002-S21F2) is expressed in XtenCHO. This receptor-binding domain (RBD) targeting human antibody (002-S21F2) effectively neutralizes live viral isolates of SARS-CoV-2 variants of concern (VOCs) including Alpha, Beta, Gamma, Delta, and Omicron (BA.1 and BA.2) with IC50 ranging from 0.02 – 0.05 μg/ml.
This antibody was isolated by screening COVID-19-recovered individuals who had experienced mild symptoms after the ancestral Wuhan strain (WA.1) transmission in the year 2020. Antibody 002-S21F2 was the most potent mAb that neutralized live SARS-CoV-2 WA.1 . Antibody 002-S21F2 has unique genetic features distinct from any reported SARS-CoV-2 mAbs. Structural studies of the full-length IgG in complex with spike trimers (Omicron and WA.1) reveal that 002-S21F2 recognizes an epitope on the outer face of RBD (class-3 surface), outside the ACE2 binding motif which enable it to overcome mutations found in the Omicron variants.

Entrez Symbol:

S

Uniprot Name:

Spike glycoprotein

Uniprot ID:

P0DTC2

Data PDF:

DVV00332

Purity:

> 95% by reducing and non-reducing SDS-PAGE

Specificity:

Recognizes SARS-CoV-2 Spike-RBD, as well as receptor-binding motif (RBM) across sarbecoviruses related to SARS-CoV-2; potential to neutralize the virus in vitro, including variant Omicron. S2E12 competitively blocks ACE2 attachment.

Host Species:

Human

Immunogen:

Derived from a convalescing COVID-19 patient's plasma

Isotype:

IgG1

Clone:

002-S21F2

Formulation:

Sterile-filtered 0.01M PBS, pH 7.4 preservative free
Endotoxin: 0.01 EU/µg by LAL analysis

Purification:

Protein A affinity chromatography

Application:

FS , Neutralizing

Application notes:

BA.2 RBD: 19.21 ng/ml
Omicron RBD: 26.48 ng/ml
XBB RBD: 6.862 mg/ml

Storage/Stability:

Stable at 2-8°C for 1 week or for up to 1 year at -20°C to -80°C. It is recommended to prepare single-use aliquots of undiluted product and store -20°C to -80°C.