Cat. No.:
CSI12858C
pdf (datasheet)
Description:
Streptavidin is a homotetrameric protein secreted by Streptomyces avidinii which binds firmly to biotin. Streptavidin is widely used in molecular biology because of its unique high affinity for biotin. The dissociation constant (Kd) of the biotin-streptavidin complex is about ~10-15 mol/L. The strong affinity recognition of biotin and biotinylated molecules has made streptavidin one of the most important components in diagnostics and laboratory kits. The streptavidin/biotin system has one of the biggest free energies of association yet observed for noncovalent binding of a protein and small ligand in aqueous solution (K_assoc = 10-14 mol/L). The complexes are also extremely stable over a wide range of temperature and pH.
Uniprot Name:
Streptavidin
Formulation:
Lyophilized from 10 mM potassium phosphate buffer, pH 6.5.
Purity:
>95% by SDS-PAGE and HPLC analyses.
Specific Activity:
> 17 U/mg (1 unit binds 1 ug D-biotin)
Storage/Stability:
Store at -20 °C to -80 °C.
Application notes:
Streptavidin may be used to visualize biotin conjugated molecules in ELISA, blotting and histological techniques.
