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Recombinant Lysostaphin

Molecule:

Lysostaphin

Synonyms:

Glycyl-glycine endopeptidase.

Cat. No.:

CRL309C pdf (datasheet)

Quantity/Size:

10 mg

Price:

$815.00 BUY

Description:

Lysostaphin, an endopeptidase which is capable of cleaving the cross-linking pentaglycine bridges in the cell wall of Staphylococci, is an extremely potent anti-staphylococcal agent. Lysostaphin is used as a research and diagnostic tool. Because it lyses staphylococci efficiently, it is widely used when preparing staphylococcal DNA or other cellular components for genetic and biochemical studies, and for the preparation of protoplasts for transformation.

Entrez Symbol:

lss

Uniprot ID:

P10547

Data PDF:

CRL309

Source:

E. coli

Molecular Weight:

26.9 kDa

Formulation:

Lyophilized from a sterile-filtered solution without additives.

Purity:

> 97% by RP-HPLC analysis

Biological Activity:

Determined by the decrease in turbidity of a suspension of heat-killed Staphylococcus aureus at pH 8.0, 30°C.

Specific Activity:

> 3,500 U/mg

Reconstitution:

Centrifuge vial prior to opening. Reconstitute in 20 mM sodium acetate, pH 4.5. The solution can then be further diluted in other aqueous buffers.

Storage/Stability:

Store in working aliquots at -80°C.

Application notes:

Lysostaphin has optimal stability in the range of pH 4.5, and optimal activity in the range of pH 8.0.
Recommended stock solution: 10 mg/ml in 20 mM sodium acetate, pH 4.5.
Recommended reaction buffer: 200 mM Tris-HCl pH 8.0.
EDTA is inhibitory for zinc enzyme lysostaphin.