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Recombinant Rat Stem Cell Factor

Molecule:

SCF

Synonyms:

c-Kit Ligand, KL, Steel Factor, MGF

Species:

Rat

Cat. No.:

CRS202C pdf (datasheet)

Quantity/Size:

1.0 mg

Price:

$3,215.00 / Special Price: $2,750.00 BUY

Description:

Recombinant Rat Stem Cell Factor

Data PDF:

CRS202

Source:

E. coli

Molecular Weight:

Approximately 18.5 kDa, a single non-glycosylated polypeptide chain containing 165 amino acids.

Formulation:

Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.

Purity:

>95% by SDS-PAGE and HPLC analyses.

Biological Activity:

Fully biologically active when compared to standard. The ED50 determined by a cell proliferation assay using human TF-1 cells is less than 20 ng/ml.

Specific Activity:

> 5 x 105 IU/mg

Endotoxin Level:

Less than 1EU/μg as determined by LAL method.

Amino Acid Sequence:

MQEICRNPVT DNVKDITKLV ANLPNDYMIT LNYVAGMDVL PSHCWLRDMV THLSVSLTTL LDKFSNISEG LSNYSIIDKL GKIVDDLVAC MEENAPKNVK ESLKKPETRN FTPEEFFSIF NRSIDAFKDF MVASDTSDCV LSSTLGPEKD SRVSVTKPFM LPPVA

Reconstitution:

Centrifuge vial prior to opening. Add sterile distilled water or aqueous buffer to a concentration of 0.1-1.0 mg/ml. Further dilutions should be made in appropriate buffered solutions.

Storage/Stability:

This lyophilized preparation is stable at 2-4°C, but should be kept desiccated at -20°C for long term storage. Upon reconstitution, the preparation is stable for up to one week at 2 -4°C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20°C to -80°C. Avoid repeated freeze/thaw cycles.