Synonyms:
OB Protein, Obesity Protein, OBS, Obesity factor.
Description:
Recombinant Rat Leptin
Molecular Weight:
16.3 kDa (147 aa) monomer
Formulation:
Lyophilized from a sterile filtered solution containing 0.1% TFA.
Purity:
≥ 95.0% by Reducing and Non-reducing SDS-PAGE
Endotoxin Level:
≤ 1 EU/μg protein by kinetic LAL analysis.
Amino Acid Sequence:
MVPIHKVQDD TKTLIKTIVT RINDISHTQS VSARQRVTGL DFIPGLHPIL SLSKMDQTLA VYQQILTSLP SQNVLQIAHD LENLRDLLHL LAFSKSCSLP QTRGLQKPES LDGVLEASLY STEVVALSRL QGSLQDILQQ LDLSPEC
Reconstitution:
Centrifuge vial prior to opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at a concentration of 0.1 mg/mL, which can be further diluted into other aqueous solutions.
Storage/Stability:
Store as supplied at -20°C to -80°C for up to 1 year. Upon reconstitution, store at 2-8°C for up to 1 month or prepare working aliquots and store at -20°C to -80°C for up to 3 months. It is recommended that a carrier protein such as 0.1% HSA or BSA is added for long term storage. Avoid repeated freeze-thaw cycles.
