Synonyms:
Interleukin-5, IL-5, B-cell growth factor II, BCGF-II, Cytotoxic T-lymphocyte inducer, Eosinophil differentiation factor, T-cell replacing factor, TRF
Description:
IL-5, also named B-cell differentiation factor I, eosinophil differentiation factor and TRF, is belonging to the cytokine family and the IL-5 gene is in close proximity to the genes encoding IL-3, IL-4, and granulocyte-macrophage colony-stimulating factor (GM-CSF), which are often co-expressed in TH2 cells. Through binding to the IL-5 receptor, IL-5 stimulates B cell growth and increases immunroglobulin secretion. It is also a key mediator in eosinophil activation. Interleukin-5 has long been associated with the cause of several allergic diseases including allergic rhinitis and asthma. Rat IL-5 is a 132-amino acid (115 in human, 133 in the mouse) -long TH2 cytokine that is part of the hematopoietic family. Unlike other members of this cytokine family (namely IL-3 and GM-CSF), this glycoprotein in its active form is a homodimer.
Uniprot Name:
Interleukin-5
Molecular Weight:
26.2 kDa (2 x 113 aa) disulfide-linked homodimer
Formulation:
Lyophilized from sterile filtered PBS, pH 7.4
Purity:
> 98 % by SDS-PAGE and HPLC analyses.
Biological Activity:
ED50 < 0.5 ng/ml, determined by a cell proliferation assay using human TF-1 cells.
Specific Activity:
> 2.0 × 10e6 IU/mg
Endotoxin Level:
< 1 EU/µg as determined by LAL method.
Amino Acid Sequence:
MEIPMSTVVK ETLIQLSTHR ALLTSNETMR LPVPTHKNHQ LCIGEIFQGL DILKNQTVRG GTVEILFQNL SLIKKYIDGQ KEKCGEERRK TRHFLDYLQE FLGVMSTEWA MEV
Storage/Stability:
Store unopened at -20°C to -80°C for up to 1 year.
