Synonyms:
Interleukin-2, aldesleukin, T cell growth factor
Description:
Recombinant Rat IL-2
Uniprot Name:
Interleukin-2
Molecular Weight:
15.6 kDa
Physical State:
Lyophilized
Formulation:
Lyophilized from a sterile-filtered aqueous solution containing 0.1% Trifluoroacetic acid
Purity:
>95 % by reducing and non-reducing SDS-PAGE
Biological Activity:
ED50 ≤ 2 ng/ml as determined by a cell proliferation assay using murine CTLL-2
Specific Activity:
≥ 5.0 × 10e5 U/mg
Endotoxin Level:
≤1 EU/µg of protein by kinetic LAL analysis.
Amino Acid Sequence:
MAPTSSPAKE TQQHLEQLLL DLQVLLRGID NYKNLKLPMM LTFKFYLPKQ ATELKHLQCL ENELGALQRV LDLTQSKSFH LEDAGNFISN IRVTVVKLKG SENKFECQFD DEPATVVEFL RRWIAICQSI ISTMTQ
Reconstitution:
Centrifuge vial prior to opening. Add sterile 10 mM HCl to a concentration of 0.1 mg/ml. DO NOT VORTEX. Allow several minutes for complete reconstitution. Further dilutions should be made in appropriate buffered solutions.
Storage/Stability:
Store as supplied at -20°C to -80°C for up to 1 year. Upon reconstitution, prepare working aliquots and store at -20°C to -80°C. It is recommended that a carrier protein such as 0.1% HSA or BSA is added for long term storage. Avoid repeated freeze-thaw cycles.
