Recombinant Rat GGTase-I, alpha and beta subunit, GST-tag

Species:

Rat

Cat. No.:

CSI11668 pdf (datasheet)

Quantity/Size:

50 μg

Price:

$720.00 BUY

Description:

GGTase-I (Geranylgeranyltransferase-I) catalyzes the transfer of the farnesyl and geranylgeranyl groups from farnesyl and geranylgeranyl-diphosphate to proteins containing a C-terminal CaaX motif, where ’C’ is a conserved cysteine that is the site of farnesyl modification, ’a’ is usually an aliphatic amino acid, and ’X’ is leucine or phenylalanine.

Farnesyltransferase (FT) and GGTase-I are closely related, sharing a common alpha subunit and 30% identity in their beta subunits.

The GST-Tag facilitates the protein`s application in typical GST pull-down assays.

Uniprot Name:

Geranylgeranyl transferase type-1 subunit beta

Uniprot ID:

P53610

Data PDF:

CSI11668

Molecular Weight:

alpha subunit: 44 kDa
beta subunit: 38 kDa

Formulation:

25 mM HEPES, 25 mM NaCl, 5 mM DTT, pH 7.2

Purity:

> 90% by SDS-PAGE

Biological Activity:

1 pmol of GGTase-I will transfer 5 pmol of Farnesyl to RhoA in 15 min at 37°C.

Storage/Stability:

Store at -80°C for up to 1 year. Upon initial thaw, prepare aliquots and store at -80°C. Avoid freeze / thaw cycles.