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Recombinant Rat FGF2/FGF basic

Molecule:

FGF2/FGF-basic

Synonyms:

HBGF-2; basic fibroblast growth factor; heparin-binding growth factor 2,Fgf-2, bFGF

Species:

Rat

Cat. No.:

CSI20131A pdf (datasheet)

Quantity/Size:

10 μg

Price:

$190.00 BUY

Description:

Recombinant Rat FGF2/FGF basic is a single non-glycosylated polypeptide chain containing 145 amino acids.

Background: Rat FGF-basic, encoded by the FGF2 gene, is a member of the fibroblast growth factor (FGF) family. Fibroblast growth factor was found in pituitary extracts in 1973 and then tested in a bioassay that caused fibroblasts to proliferate. After further fractionating the extract using acidic and basic pH, two different forms have isolated that named "acidic fibroblast growth factor" (FGF1) and "basic fibroblast growth factor" (FGF2). Mouse FGF-basic shares 95 % amino acid sequence identity with human FGF-basic. Affinity between FGF-basic and its receptors can be increased by heparin or heparan sulfate proteoglycan. FGF-basic plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. FGF-basic are also involved in a variety of biological processes, including embryonic development , morphogenesis, tissue repair, tumor growth and invasion. Additionally, FGF-basic is frequently used as a critical component of cell culture medium, e.g., human embryonic stem cell culture medium, serum-free culture systems.

Entrez Symbol:

Fgf2

Data PDF:

CSI20131

Source:

E. coli

Molecular Weight:

~16.4 kDa

Formulation:

Lyophilized from a 0.2μm filtered solution in PBS, pH 7.4.

Purity:

>98% by SDS-PAGE and HPLC analyses.

Biological Activity:

Fully biologically active when compared to standard. The ED50 determined by a cell proliferation assay using murine NR6R/3T3 cells is less than 0.2 ng/ml.

Specific Activity:

≥5 x 106 IU/mg

Endotoxin Level:

Less than 1EU/μg of rRtbFGF as determined by LAL method.

Amino Acid Sequence:

PALPEDGGGA FPPGHFKDPK RLYCKNGGFF LRIHPDGRVD GVREKSDPHV KLQLQAEERG VVSIKGVCAN RYLAMKEDGR LLASKCVTEE CFFFERLESN NYNTYRSRKYSSWYVALKRT GQYKLGSKTG PGQKAILFLP MSAKS

Reconstitution:

Centrifuge vial prior to opening. Add sterile distilled water or aqueous buffer to a concentration of 0.1-1.0 mg/mL. Further dilutions should be made in appropriate buffered solutions.

Storage/Stability:

This lyophilized preparation is stable at 2-4°C, but should be kept desiccated at -20°C for long term storage. Upon reconstitution, the preparation is stable for up to one week at 2-4°C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20°C to -80°C. Avoid repeated freeze/thaw cycles.