Recombinant Protein A/G, His Tag

Synonyms:

Immunoglobulin G-binding protein A

Cat. No.:

CSI20106A pdf (datasheet)

Quantity/Size:

1 mg

Price:

$195.00 BUY

Description:

The recombinant Protein A/G consists of 8 IgG-binding domains EDABC-C1C2C3, which corresponds to the Protein A and G domains that are included in the recombinant sequence. The Protein A portion is from Staphylococcus aureus segments E, D, A, B and C. The Protein G portion is from Streptococcus segments C1, C2 and C3. The binding capacity of recombinant Protein A/G is broader than either Protein A or Protein G alone.

Uniprot Name:

Immunoglobulin G-binding protein A

Uniprot ID:

P02976

Data PDF:

CSI20106

Source:

E. coli

Molecular Weight:

Recombinant Protein A/G, with a C-terminal 6X-His tag, is a single non-glycosylated protein of 513 aa and a predicted MW of 56.9 kDa.

Formulation:

Lyophilized from a sterile-filtered solution.

Purity:

>96% by SDS-PAGE and RP-HPLC analyses

Endotoxin Level:

< 0.1EU/ug of Protein A/G, His tag as determined by LAL method.

Amino Acid Sequence:

MNAAQHDEAQ QNAFYQVLNM PNLNADQRNG FIQSLKDDPS QSANVLGEAQ KLNDSQAPKA DAQQNNFNKD QQSAFYEILN MPNLNEAQRN GFIQSLKDDP SQSTNVLGEA KKLNESQAPK ADNNFNKEQQ NAFYEILNMP NLNEEQRNGF IQSLKDDPSQ SANLLSEAKK LNESQAPKAD NKFNKEQQNA FYEILHLPNL NEEQRNGFIQ SLKDDPSQSA NLLAEAKKLN DAQAPKADNK FNKEQQNAFY EILHLPNLTE EQRNGFIQSL KDDPSVSKEI LAEAKKLNDA QAPKEEDSLE GSGSGTYKLI LNGKTLKGET TTEAVDAATA EKVFKQYAND NGVDGEWTYD DATKTFTVTE KPEVIDASEL TPAVTTYKLV INGKTLKGET TTEAVDAATA EKVFKQYAND NGVDGEWTYD DATKTFTVTE KPEVIDASEL TPAVTTYKLV INGKTLKGET TTKAVDAETA EKAFKQYAND NGVDGVWTYD DATKTFTVTE KLAAALEHHH HHH

Reconstitution:

Centrifuge vial prior to opening. Dissolve in distilled water or saline.

Storage/Stability:

Store as supplied at -20°C to -80°C for up to 1 year. Upon reconstitution as directed, stable for up to 1 month at 2-8°C or prepare working aliquots and store at -20°C to -80°C for up to 3 months. Avoid repeated freeze-thaw cycles.