Synonyms:
Interleukin-4, B-cell stimulatory factor-1, BSF-1, BCDF, BCGF
Description:
Recombinant Porcine IL-4
Uniprot Name:
Interleukin-4
Molecular Weight:
12.7 kDa (110 aa) monomer
Formulation:
Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 10 mM sodium phosphate, pH 7.5
Purity:
≥ 95% by reducing and non-reducing SDS-PAGE
Biological Activity:
ED50 ≤ 2 ng/ml, determined by TF-1 cell proliferation.
Specific Activity:
≥ 5.0 x 105 units/mg
Endotoxin Level:
≤ 1 EU/µg by kinetic LAL
Amino Acid Sequence:
MHKCDITLQE IIKTLNILTA RKNSCMELPV TDVFAAPENT TEKETFCRAS TVLRHIYRHH TCMKSLLSGL DRNLSSMANM TCSVHEAKKS TLKDFLERLK TIMKEKYSKC
Reconstitution:
Centrifuge vial before opening. Add sterile water at 0.1 mg/ml. Suspend the product by gently pipetting the above recommended solution down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution. For prolonged storage, dilute to working aliquots in a 0.1% BSA solution, store at -80°C and avoid repeat freeze thaws.
Storage/Stability:
Lyophilized product is stable at room temperature for shipping purposes. Upon receipt, store at -20°C to -80°C for up to 1 year. Upon reconstitution, the preparation is stable for up to one month at 2-8°C. For long term storage, prepare working aliquots and store at -20 to -80°C. For maximal stability, dilute to working aliquots in a 0.1% BSA solution. Avoid repeated freeze-thaw cycles.
