Recombinant Mouse Soluble RANK Ligand

Molecule:

RANKL

Synonyms:

Tumor necrosis factor ligand superfamily member 11, Receptor activator of nuclear factor kappa-B ligand, RANKL, CD254, Osteoclast differentiation factor, ODF, TNF-related activation-induced cytokine, TRANCE

Species:

Mouse

Cat. No.:

CRR101D pdf (datasheet)

Quantity/Size:

100 μg

Price:

$820.00 BUY

Description:

Recombinant Mouse Soluble RANK Ligand *am

Entrez Symbol:

Tnfsf11

Uniprot Name:

Tumor necrosis factor ligand superfamily member 11

Uniprot ID:

O35235

Data PDF:

CRR101D

Source:

E. coli

Molecular Weight:

Monomer, 19.5 kDa (175 aa)

Formulation:

Lyophilized from 10 mM sodium phosphate, 20 mM sodium chloride, pH 7.5.

Purity:

≥ 95% by reducing and non-reducing SDS-PAGE

Biological Activity:

ED50 ≤ 50 ng/mL determined by activity of RAW-Blue™ cells. This product also demonstrates TNF-alpha production in RAW cells (typical ED50 is 50-75 ng/mL) and Osteoclast formation (first detectable at 50 ng/mL).

Specific Activity:

≥ 2.0 x 104 units/mg

Endotoxin Level:

≤1 EUs/µg by kinetic LAL analysis.

Amino Acid Sequence:

MPAMMEGSWL DVAQRGKPEA QPFAHLTINA ASIPSGSHKV TLSSWYHDRG WAKISNMTLS NGKLRVNQDG FYYLYANICF RHHETSGSVP TDYLQLMVYV VKTSIKIPSS HNLMKGGSTK NWSGNSEFHF YSINVGGFFK LRAGEEISIQ VSNPSLLDPD QDATYFGAFK VQDID

Reconstitution:

Centrifuge vial prior to opening. Add sterile distilled water to reconstitute to a recommended concentration of 0.1 mg/mL and gently pipette solution up and down sides of vial. DO NOT VORTEX. Allow several minutes for complete reconstitution.

Storage/Stability:

Store as supplied at -20°C to -80°C for up to 1 year. Upon reconstitution, prepare working aliquots and store at -20°C to -80°C. It is recommended that a carrier protein such as 0.1% HSA or BSA is added for long term storage. Avoid repeated freeze-thaw cycles.