Cat. No.:
CSI20130B
pdf (datasheet)
Description:
Noggin belongs to a group of diffusible proteins which bind to ligands of the TGF-β family and regulate their activity by inhibiting their access to signaling receptors. Noggin was originally identified as a BMP-4 antagonist whose action is critical for proper formation of the head and other dorsal structures. Noggin has been shown to modulate the activities of other BMPs including BMP-2,-7,-13, and -14. Targeted deletion of Noggin in mice results in prenatal death and recessive phenotype displaying a severely malformed skeletal system. Conversely, transgenic mice over-expressing Noggin in mature osteoblasts display impaired osteoblastic differentiation, reduced bone formation, and severe osteoporosis.
Mature mouse Noggin shares 99 % and 83 % a.a. sequence identity with human and Xenopus Noggin, respectively.
Molecular Weight:
~46.4 kDa (two 206 aa) disulfide linked homodimer
Formulation:
Lyophilized from a sterile filtered concentrated solution in 30% acetonitrile, 0.1% TFA
Purity:
>95% by SDS-PAGE and HPLC
Biological Activity:
ED50 < 2.0 ng/ml, determined by inhibition of BMP-4-induced (5 ng/ml) alkaline phosphatase production by murine ATDC5 cells.
Specific Activity:
> 5.0 x 105 IU/mg.
Endotoxin Level:
<1 EU/μg by LAL analysis
Amino Acid Sequence:
MQHYLHIRPAPSDNLPLVDLIEHPDPIFDPKEKDLNETLLRSLLGGHYDPGFMATSPPEDRPGGGGGPAGGAEDLAELDQLLRQRPSGAMPSEIKGLEFSEGLAQGKKQRLSKKLRRKLQMWLWSQTCPVLYAWNDLGSRFWPRYVKVGSCFSKRSCSV PEGMVCKPSK SVHLTVLRWR CQRRGGQRCG WIPIQYPIIS ECKCSC
Reconstitution:
Centrifuge vial prior to opening. Reconstitute in 10 mM HAc to a concentration less than 0.25 mg/mL. Further dilutions should be made in appropriate buffered solutions.
Storage/Stability:
Store at -20°C to -80°C for up to 1 year.
