Recombinant Mouse Multimeric APRIL, FLAG Tag

Synonyms:

Tumor necrosis factor ligand superfamily member 13, A proliferation-inducing ligand, APRIL, CD256, Adiponectin, Adipocyte complement-related 30 kDa protein, ACRP30

Species:

Mouse

Cat. No.:

CRM032 pdf (datasheet)

Quantity/Size:

10 μg

Price:

$424.00 BUY

Description:

APRIL is a cytokine that belongs to the TNF superfamily and binds to TACI and BCMA. It is implicated in the regulation of tumor cell growth, is involved in monocyte/macrophage-mediated immunological processes and functions as an important survival factor for plasmablasts and bone marrow plasma cells. Multimeric APRIL is a high activity construct in which two trimeric APRIL ligands are artificially linked via the collagen domain of ACRP30. This construct very effectively stimulates proliferation B cell. A basic amino acid sequence (QKQKKQ) close to the N-terminus of APRIL is required for binding to negatively charged sulfated glycosaminoglycan side chains of proteoglycans. Proteoglycans mediate binding of APRIL to tumor cells as well as primary lymphoid cells.

Entrez Symbol:

Tnfsf13, Adipoq

Uniprot Name:

Tumor necrosis factor ligand superfamily member 13, Adiponectin

Uniprot ID:

Mouse APRIL: Q9D777
Mouse ACRP30 (adiponectin): Q60994

Data PDF:

CRM032x

Source:

HEK293 cells

Molecular Weight:

~34 kDa by SDS-PAGE

Formulation:

Lyophilized from PBS with 0.5% trehalose

Purity:

≥95% (SDS-PAGE)

Biological Activity:

Stimulates B cell proliferation.

Endotoxin Level:

<0.02 EU/μg purified protein as determined by LAL test

Amino Acid Sequence:

The extracellular domain of mouse APRIL (aa 88-232) is fused at the N-terminus to mouse collagen domain of ACRP30headless (aa18-111) and a FLAG tag.

Storage/Stability:

Store at -20°C to -80°C

Application notes:

Binds to human and mouse BCMA and TACI. Binds to proteoglycans.