Synonyms:
Interleukin-4, BSF-1, IL-4, B cell Stimulating Factor, BCDF, BCGF
Description:
Recombinant Mouse IL-4
Uniprot Name:
Interleukin-4
Molecular Weight:
13.7 kDa (121 aa)
Physical State:
Lyophilized
Formulation:
Sterile-filtered aqueous solution containing 0.1% Trifluoroacetic Acid (TFA).
Purity:
>95%, by reducing and non-reducing SDS-PAGE.
Biological Activity:
ED50 < 20 ng/ml, as determined by the dose-dependent proliferation of Mouse HT-2 cells calibrated against recombinant mouse IL-4 WHO International Standard (NIBSC code: 91/656). .
Specific Activity:
≥ 5 x 10e4 IU/mg
Endotoxin Level:
≤1 EU/µg of protein by kinetic LAL analysis
Amino Acid Sequence:
MHIHGCDKNH LREIIGILNE VTGEGTPCTE MDVPNVLTAT KNTTESELVC RASKVLRIFY LKHGKTPCLK KNSSVLMELQ RLFRAFRCLD SSISCTMNES KSTSLKDFLE SLKSIMQMDY S
Reconstitution:
Centrifuge vial prior to opening. Add sterile distilled water to a concentration of 0.1 mg/ml. DO NOT VORTEX. Allow several minutes for complete reconstitution. Further dilutions should be made in appropriate buffered solutions.
Storage/Stability:
Store at -20°C to -80°C for up to 1 year.
