Synonyms:
interleukin-3, mast cell growth factor, mast-cell growth factor, P-cell stimulating factor, P-cell-stimulating factor, hematopoietic growth factor, multilineage-colony-stimulating factor, multipotential colony-stimulating factor
Description:
Recombinant Mouse IL-3, *am
Molecular Weight:
15.2 kDa (135 aa) monomer
Physical State:
Lyophilized
Formulation:
Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 0.1% Trifluoroacetic Acid (TFA)
Purity:
> 95.0% by reducing and non-reducing SDS-PAGE
Biological Activity:
ED50 ≤250 pg/ml, as determined by the dose-dependent proliferation of murine M-NFS-60 cells.
Specific Activity:
≥ 4.0 x 10e6 units/mg
Endotoxin Level:
<1 EU/µg, by kinetic LAL.
Amino Acid Sequence:
MDTHRLTRTL NCSSIVKEII GKLPEPELKT DDEGPSLRNK SFRRVNLSKF VESQGEVDPE DRYVIKSNLQ KLNCCLPTSA NDSALPGVFI RDLDDFRKKL RFYMVHLNDL ETVLTSRPPQ PASGSVSPNR GTVEC
Reconstitution:
Centrifuge vial prior to opening. Add sterile distilled water or aqueous buffer to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤-20°C. Further dilutions should be made in appropriate buffered solutions.
Storage/Stability:
Store at -20°C to -80°C for up to 1 year.