Synonyms:
IL-2, lymphokine, aldesleukin, T cell growth factor
Description:
Recombinant Mouse IL-2
Uniprot Name:
Interleukin-2
Molecular Weight:
Monomer, 17.4 kDa (150 aa)
Physical State:
Lyophilized
Formulation:
Lyophilized from sterile filtered aqueous solution containing 10 mM sodium citrate, pH 4.0.
Purity:
≥95% by nonreducing and reducing SDS-PAGE
Biological Activity:
ED50 < 5 ng/ml, as determined by proliferation assay of murine CTLL-2 cells.
Endotoxin Level:
≤ 1 EU/µg, by kinetic LAL
Amino Acid Sequence:
MAPTSSSTSS STAEAQQQQQ QQQQQQQHLE QLLMDLQELL SRMENYRNLK LPRMLTFKFY LPKQATELKD LQCLEDELGP LRHVLDLTQS KSFQLEDAEN FISNIRVTVV KLKGSDNTFE CQFDDESATV VDFLRRWIAF CQSIISTSPQ
Reconstitution:
Centrifuge vial prior to opening. Add sterile 10mM acetic acid to a concentration of 0.1 mg/mL. DO NOT VORTEX. Allow several minutes for complete reconstitution. Further dilutions should be made in appropriate buffered solutions.
Storage/Stability:
Store as supplied at -20 °C to -80 °C for up to 1 year. Upon reconstitution, the preparation is stable for up to one month at 2-8°C. For long term storage, freeze in working aliquots containing 0.1% BSA at -20 °C to -80 °C. Avoid repeated freeze-thaw cycles.
