Synonyms:
IL-13, T-cell activation protein P600
Description:
Recombinant Mouse IL-13
Uniprot Name:
Interleukin-13
Molecular Weight:
12.3 kDa (111 aa) monomer
Formulation:
Lyophilized from a sterile filtered aqueous solution containing 0.1% Trifluoroacetic Acid.
Purity:
≥ 95% by reducing and non-reducing SDS-PAGE
Biological Activity:
ED50 ≤ 10 ng/ml, determined by a cell proliferation assay using the TF-1 cell line.
Specific Activity:
≥ 1.0 × 10e5 U/mg
Endotoxin Level:
≤ 1 EU/μg determined by kinetic LAL analysis.
Amino Acid Sequence:
MPVPRSVSLP LTLKELIEEL SNITQDQTPL CNGSMVWSVD LAAGGFCVAL DSLTNISNCN AIYRTQRILH GLCNRKAPTT VSSLPDTKIE VAHFITKLLS YTKQLFRHGP FMPVPRSVSLP LTLKELIEEL SNITQDQTPL CNGSMVWSVD LAAGGFCVAL DSLTNISNCN AIYRTQRILH GLCNRKAPTT VSSLPDTKIE VAHFITKLLS YTKQLFRHGP F
Reconstitution:
Centrifuge vial prior to opening. Add sterile 20 mM HCl to a concentration of 0.1 mg/mL and gently pipet the solution up and down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution.
Storage/Stability:
Store as supplied at -20°C to -80°C for up to 1 year. Upon reconstitution, prepare working aliquots and store at -20°C to -80°C. It is recommended that a carrier protein such as 0.1% HSA or BSA is added for long term storage. Avoid repeated freeze-thaw cycles.