Synonyms:
IL-10, Cytokine Synthesis Inhibitory Factor, CSIF
Description:
Recombinant Mouse IL-10 *am
Uniprot Name:
Interleukin-10
Molecular Weight:
18.9 kDa (161 aa) noncovalent homodimer
Physical State:
Lyophilized
Formulation:
Lyophilized from a sterile filtered solution containing 10 mM sodium phosphate, pH 7.5
Purity:
>95% by reducing and non-reducing SDS-PAGE
Biological Activity:
ED50≤ 5 ng/ml, determined by dose-dependent proliferation of mouse MC/9 cells.
Specific Activity:
≥ 2.0 x 10e5 U/mg
Endotoxin Level:
< 1.0 EU/µg by kinetic LAL analysis
Amino Acid Sequence:
MSRGQYSRED NNCTHFPVGQ SHMLLELRTA FSQVKTFFQT KDQLDNILLT DSLMQDFKGY LGCQALSEMI QFYLVEVMPQ AEKHGPEIKE HLNSLGQKLK TLRMRLRRCH RFLPCENKSK AVEQVKSDFN KLEDQGVYKA MNEFDIFINC IEAYMMIKMK S
Reconstitution:
Centrifuge vial prior to opening. Add sterile distilled water to a concentration of 0.1 mg/mL and gently pipette the solution up and down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution.
Storage/Stability:
Store as supplied at -20°C to -80°C for up to 1 year. Upon reconstitution, prepare working aliquots and store at -20°C to -80°C. It is recommended that a carrier protein such as 0.1% HSA or BSA is added for long term storage. Avoid repeated freeze-thaw cycles.
