Recombinant Mouse GM-CSF, Animal Free

Molecule:

CSF2

Synonyms:

Granulocyte-macrophage colony-stimulating factor, GM-CSF, Colony-stimulating factor, CSF

Species:

Mouse

Cat. No.:

CRG101B-AF pdf (datasheet)

Quantity/Size:

20 µg

Price:

$260.00 BUY

Description:

Granulocyte-macrophage colony-stimulating factor (GM-CSF) is hematopoietic growth factor produced by endothelial cells, monocytes, fibroblasts, and T cells. GM-CSF stimulates the production of neutrophilic granulocytes, macrophages, and mixed granulocyte-macrophage colonies from bone marrow cells. GM-CSF promotes immune system development and regulates neutrophil function during infection.
Human and mouse GM-CSF show no cross-reactivity.

Manufactured in an Animal-Free facility, without animal-derived materials.

Uniprot Name:

Granulocyte-macrophage colony-stimulating factor

Uniprot ID:

P01587

Data PDF:

CRG101-AF

Source:

E. coli

Molecular Weight:

14.3 kDa (125 aa) monomer

Formulation:

Lyophilized from sterile filtered 10 mM acetic acid.

Purity:

≥ 95% by reducing and non-reducing SDS-PAGE

Biological Activity:

ED50 ≤ 50 pg/ml, determined by the dose-dependent stimulation of the proliferation of mouse FDC-P1 cells.

Endotoxin Level:

< 0.10 EU/µg by kinetic LAL analysis

Amino Acid Sequence:

MAPTRSPITV TRPWKHVEAI KEALNLLDDM PVTLNEEVEV VSNEFSFKKL TCVQTRLKIF EQGLRGNFTK LKGALNMTAS YYQTYCPPTP ETDCETQVTT YADFIDSLKT FLTDIPFECK KPVQK

Reconstitution:

Centrifuge vial prior to opening. Add sterile distilled water to reconstitute to a recommended concentration of 0.1 mg/mL and gently pipet solution up and down sides of vial. DO NOT VORTEX. Allow several minutes for reconstitution.

Storage/Stability:

Store as supplied at -20°C to -80°C for up to 1 year. Upon reconstitution, prepare working aliquots and store at -20°C to -80°C. It is recommended that a carrier protein such as 0.1% HSA or BSA is added for long term storage. Avoid repeated freeze-thaw cycles.