Synonyms:
Granulocyte-macrophage colony-stimulating factor, GM-CSF, Colony-stimulating factor, CSF
Description:
Granulocyte-macrophage colony-stimulating factor (GM-CSF) is hematopoietic growth factor produced by endothelial cells, monocytes, fibroblasts, and T cells. GM-CSF stimulates the production of neutrophilic granulocytes, macrophages, and mixed granulocyte-macrophage colonies from bone marrow cells. GM-CSF promotes immune system development and regulates neutrophil function during infection.
Human and mouse GM-CSF show no cross-reactivity.
Uniprot Name:
Granulocyte-macrophage colony-stimulating factor
Molecular Weight:
14.3 kDa (125 aa) monomer
Formulation:
Lyophilized from sterile filtered 10 mM acetic acid.
Purity:
≥ 95% by reducing and non-reducing SDS-PAGE
Biological Activity:
ED50 ≤ 50 pg/ml, determined by the dose-dependent stimulation of the proliferation of mouse FDC-P1 cells.
Endotoxin Level:
< 0.10 EU/µg by kinetic LAL analysis
Amino Acid Sequence:
MAPTRSPITV TRPWKHVEAI KEALNLLDDM PVTLNEEVEV VSNEFSFKKL TCVQTRLKIF EQGLRGNFTK LKGALNMTAS YYQTYCPPTP ETDCETQVTT YADFIDSLKT FLTDIPFECK KPVQK
Reconstitution:
Centrifuge vial prior to opening. Add sterile distilled water to reconstitute to a recommended concentration of 0.1 mg/mL and gently pipet solution up and down sides of vial. DO NOT VORTEX. Allow several minutes for reconstitution.
Storage/Stability:
Store as supplied at -20°C to -80°C for up to 1 year. Upon reconstitution, prepare working aliquots and store at -20°C to -80°C. It is recommended that a carrier protein such as 0.1% HSA or BSA is added for long term storage. Avoid repeated freeze-thaw cycles.
