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Recombinant Mouse CXCL12/SDF-1 alpha

Molecule:

SDF-1 alpha

Species:

Mouse

Cat. No.:

CRS001B pdf (datasheet)

Quantity/Size:

10 μg

Price:

$240.00 BUY

Description:

Stromal cell-derived factor-1 alpha (SDF-1α), the alpha splice variant of the CXCL12 gene, is one of two SDF-1 splice variants made by a wide variety of cells upon stimulation by inflammatory cytokines such as TNF, IL-1, and LPS. SDF-1α signals through the G protein-coupled receptor CXCR4 to recruit activated leukocytes.
Human and mouse SDF-1α share 99% sequence identity.

Uniprot Name:

Stromal cell-derived factor 1

Uniprot ID:

Q4FJL5

Data PDF:

CRS001

Source:

E. coli

Molecular Weight:

8 kDa (68 aa), monomer
Under non-reducing conditions, samples prepared at higher working concentrations produce a band of ~16 kDa on an SDS PAGE gel, which may represent dimer formation.

Formulation:

Lyophilized from a sterile-filtered solution containing 1% Trifluoroacetic acid (TFA)

Purity:

≥95% by reducing and non-reducing SDS-PAGE

Biological Activity:

Activity data are unavailable at this time.

Endotoxin Level:

≤1EU/μg, determined by kinetic LAL analysis

Amino Acid Sequence:

KPVSLSYRCP CRFFESHIAR ANVKHLKILN TPNCALQIVA RLKNNNRQVC IDPKLKWIQE YLEKALNK

Reconstitution:

Centrifuge vial prior to opening. Add sterile distilled water to a concentration of 0.1 mg/mL and gently pipette the solution up and down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution.

Storage/Stability:

Store as supplied at -20°C to -80°C for up to 1 year. Upon reconstitution, store at 2-8°C for up to 1 month or prepare working aliquots and store at -20°C to -80°C for up to 3 months. It is recommended that a carrier protein such as 0.1% HSA or BSA is added for long term storage. Avoid repeated freeze-thaw cycles.