Synonyms:
Ciliary neurotrophic factor
Description:
Ciliary neurotrophic factor (CNTF) is a hormone whose actions appear to be restricted to the nervous system where it promotes neurotransmitter synthesis and neurite outgrowth in certain neuronal populations. CNTF was initially identified as a trophic factor for embryonic chick ciliary para-sympathetic neurons in culture. Furthermore, the protein is also a potent survival factor for neurons and oligodendrocytes and may be relevant in reducing tissue destruction during inflammatory attacks. In addition, CNTF is useful for treatment of motor neuron disease and it could reduce food intake without causing hunger or stress. Recombinant mouse CNTF shares 82% and 95% a.a. sequence identity with human and rat CNTF.
Uniprot Name:
Ciliary neurotrophic factor
Molecular Weight:
22.5 kDa (197 aa) monomer
Formulation:
Lyophilized from sterile filtered 20 mM phosphate buffer, pH 7.4, 150 mM NaCl, with 5 % Trehalose, 0.02 % Tween-20
Purity:
>95% by SDS-PAGE and HPLC analyses.
Biological Activity:
ED50 < 30 ng/ml, determined by a cell proliferation assay using human TF-1 cells
Specific Activity:
> 3.3 × 104 IU/mg.
Endotoxin Level:
Ciliary neurotrophic factor, CNTF
Storage/Stability:
Store lyophilized protein at -20°C to -80°C for 1 year.
