CD27 antigen, CD27L receptor, T-cell activation antigen CD27, Tumor necrosis factor receptor superfamily member 7, CD27

Mouse

CRM534B-His pdf (datasheet)

100 µg

$450.00 BUY

CD27 is a member of the TNF-receptor superfamily limited to cells of the lymphoid lineage, and exists as both a dimeric glycoprotein on the cell surface and as a soluble protein in serum. As a type I transmembrane glycoprotein, CD27 has roles in lymphoid proliferation, differentiation, apoptosis, generation of T cell immunity, and is an apparently robust marker for normal memory B cells. It is a T and B cell co-stimulatory molecule, the activity of CD27 is governed by its TNF-like ligand CD7 on lymphocytes and dendritic cells. The CD27-CD7 interaction is required for Th1 generation responses to differentiation signals and long-term maintenance of T cell immunity, and meanwhile, plays a key role in regulating B-cell differentiation, activation and immunoglobulin synthesis.

Cd27

CD27 antigen

P41272

CRM534-His

NP_001028298.1

HEK293 cells

The rmCD27 consists of 173 aa after removal of the signal peptide with a predicted MW of 19.6 kDa and migrates at ~35 kDa in SDS-PAGE under reducing conditions, due to glycosylation.

His

Lyophilized from sterile PBS, pH 7.4

Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.

> 96 % as determined by SDS-PAGE.

Measured by its binding ability in a functional ELISA. Immobilized recombinant mouse CD27 at 2 μg/ml (100 μl/well) can bind human CD70 with a linear range of 78-1250 pg/ml.

< 1.0 EU per μg protein as determined by the LAL method.

Centrifuge vial prior to opening. Add sterile distilled water to a concentration of 0.1 mg/mL and gently pipette the solution up and down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution.

Stable for up to 1 year from date of receipt at -20°C to -80°C After reconstitution, store working aliquots at -20°C to -80°C. Avoid repeated freeze-thaw cycles.