Recombinant Mouse MIP1A/CCL3

Molecule:

CCL3

Synonyms:

MIP1-alpha, MIP-1 alpha, C-C motif chemokine, CCL3

Species:

Mouse

Cat. No.:

CRM401C pdf (datasheet)

Quantity/Size:

1.0 mg

Price:

$3,215.00 / Special Price: $2,750.00 BUY

Description:

Recombinant Mouse MIP1A/CCL3

Entrez Symbol:

Ccl3

Uniprot Name:

C-C motif chemokine

Uniprot ID:

Q5QNW0

Data PDF:

CRM401

Source:

E. coli

Molecular Weight:

7.8 kDa (69 aa) monomer

Physical State:

Lyophilized

Formulation:

Lyophilized from a sterile filtered aqueous solution containing 0.1% Trifluoroacetic Acid

Purity:

≥ 95%, determined by reducing and non-reducing SDS-PAGE

Biological Activity:

ED50 < 100 ng/ml, determined by dose-dependent chemotaxis with THP-1 cells. Product demonstrates dose-dependent chemotaxis with human PBMC.

Endotoxin Level:

≤ 1 EU/μg, by kinetic LAL analysis

Amino Acid Sequence:

APYGADTPTA CCFSYSRKIP RQFIVDYFET SSLCSQPGVI FLTKRNRQIC ADSKETWVQE YITDLELNA

Reconstitution:

Centrifuge vial prior to opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at a concentration of 0.1 mg/mL, which can be further diluted into other aqueous solutions.

Storage/Stability:

Store as supplied at -20°C to -80°C for up to 1 year. Upon reconstitution, prepare working aliquots and store at -20°C to -80°C. It is recommended that a carrier protein such as 0.1% HSA or BSA is added for long term storage. Avoid repeated freeze-thaw cycles.