Recombinant IdeS Protease (IgG specific)

Species:

Streptococcus

Cat. No.:

YXX05001B pdf (datasheet)

Quantity/Size:

1.0 mg

Price:

$1,870.00 BUY

Description:

Streptococcus pyogenes, a significant bacterial pathogen, secretes two enzymes showing remarkable specificity for IgG; EndoS and IdeS. IdeS (Immunoglobulin G-degrading enzyme of Streptococcus pyogenes) is a cysteine protease which cleaves IgG with a unique degree of specificity at a single site in the hinge region yielding F(ab’)2 and Fc fragments.

Available in BULK quantities as low endotoxin and without ProClin 300.

Concentration:

20,000 Units/mg

Uniprot Name:

Ig protease IdeS domain-containing protein

Data PDF:

YXX05001

Source:

E. coli

Molecular Weight:

37.57 kDa

Formulation:

0.01M PBS, pH 7.4, 50% glycerol, 0.05% Proclin 300

Purity:

> 90 % as determined by SDS-PAGE.

Biological Activity:

One unit will cleave ≥95% of 1μg of recombinant mAb IgG in 30 minutes at 37°C

Storage/Stability:

Store at -80°C up to 1 year

Application notes:

1. Add appropriate amount of IgG (to 5mg) in digestive juice;
2. Add IdeS protease to IgG samples: add 1 unit of IdeS per 1ug of IgG;
3. Incubate the sample at 37°C for 30-60min.
* IdeS proteases are most active in buffers at or near neutral pH. The recommended reaction buffer is 50 mM sodium phosphate and 150 mM NaCl (pH 6.6), but most common biological buffers are suitable, such as Tris or PBS. Buffers outside this pH range (such as acetate buffer) may also be suitable, but the incubation time or enzyme amount needs to be optimized according to the actual situation.