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Recombinant Human VEGF110 / VEGF-A

Synonyms:

Vascular endothelial growth factor A, VEGF-A, Vascular permeability factor, VPF

Species:

Human

Cat. No.:

CRH757B-2 pdf (datasheet)

Quantity/Size:

50 µg

Price:

$650.00 BUY

Description:

Vascular endothelial growth factor (VEGF), also known as vascular permeability factor (VPF) and VEGF-A, is a potent mediator of both angiogenesis and vasculogenesis in the fetus and adult. It is a member of the platelet-derived growth factor (PDGF)/vascular endothelial growth factor (VEGF) family and often exists as a disulfide-linked homodimer. VEGF-A protein is a glycosylated mitogen that specifically acts on endothelial cells and has various effects, including mediating increased vascular permeability, inducing angiogenesis, vasculogenesis and endothelial cell growth, promoting cell migration, inhibiting apoptosis and tumor growth. VEGF-A protein is also a vasodilator that increases microvascular permeability, thus it was originally referred to as vascular permeability factor

Entrez Symbol:

VEGFA

Uniprot Name:

Vascular endothelial growth factor A

Uniprot ID:

P15692

Data PDF:

CRH757-2

Genbank Accession No:

NP_001191313.1

Source:

HEK293 cells

Molecular Weight:

The recombinant human VEGF110 consists of 110 amino acids with a predicted molecular mass of 12.7 kDa.

Formulation:

Lyophilized from sterile PBS, pH 7.4 containing 5 % trehalose, 5% mannitol and 0.01% Tween80.

Purity:

> 95 % as determined by SDS-PAGE.

Biological Activity:

Measured in a cell proliferation assay using human umbilical vein endothelial cells (HUVEC). The ED50 for this effect is 2-10 ng/mL.

Endotoxin Level:

< 1.0 EU per μg protein as determined by the LAL method.

Reconstitution:

Centrifuge vial prior to opening. Add sterile distilled water to a concentration of 0.1 mg/mL and gently pipette the solution up and down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution.

Storage/Stability:

Stable for up to 1 year from date of receipt at -20°C to -80°C After reconstitution, store working aliquots at -20°C to -80°C. Avoid repeated freeze-thaw cycles.