Recombinant Human VEGFR-1 (D1-3) soluble

Molecule:

VEGFR1/FLT1

Synonyms:

Vascular Endothelial Growth Factor Receptor-1 domain D1-3, fms-like tyrosine kinase 1, FLT-1

Species:

Human

Cat. No.:

CRF101B pdf (datasheet)

Quantity/Size:

20 μg

Price:

$600.00 BUY

Description:

Recombinant human soluble Vascular Endothelial Growth Factor Receptor-1 domain D1-3 (sVEGFR-1D1-3) is produced as a non-chimeric protein in a monomeric form. The soluble receptor protein contains only the first 3 extracellular domains, which contain all the information necessary for binding of VEGF.

Endothelial cells express three different vascular endothelial growth factor (VEGF) receptors, belonging to the family of receptor tyrosine kinases (RTKs). They are named VEGFR-1 (Flt-1), VEGFR-2 (KDR/Flk-1), VEGFR-3 (Flt-4). Their expression is almost exclusively restricted to endothelial cells, but VEGFR-1 can also be found on monocytes, dendritic cells and on trophoblast cells. The flt-1 gene was first described in 1990. The receptor contains seven immunoglobulin-like extracellular domains, a single transmembrane region and an intracellular splited tyrosine kinase domain. Compared to VEGFR-2 the Flt-1 receptor has a higher affinity for VEGF but a weaker signaling activity. VEGFR-1 thus leads not to proliferation of endothelial cells, but mediates signals for differentiation. Interestingly a naturally occuring soluble variant of VEGFR-1 (sVEGFR-1) was found in HUVE supernatants in 1996, which is generated by alternative splicing of the flt-1 mRNA. The biological functions of sVEGFR-1 still are not clear, but it seems to be an endogenous regulator of angiogenesis, binding VEGF with the same affinity as the full-length receptor.

Entrez Symbol:

FLT1

Uniprot Name:

Vascular endothelial growth factor receptor 1

Uniprot ID:

P17948-2

EC number:

2.7.10.1

Data PDF:

CRF101

Source:

Sf9 insect cells

Molecular Weight:

45 kDa (327 aa)

Formulation:

Lyophilized from PBS

Purity:

> 90% as determined by SDS-PAGE with Coomassie stain

Biological Activity:

The activity of sVEGFR-1(D3) was determined by its ability to inhibit the VEGF-A-induced proliferation of HUVECs.

Endotoxin Level:

< 1 EU/µg

Amino Acid Sequence:

SKLKDPELSLKGTQHIMQAGQTLHLQCRGEAAHKWSLPEMVSKESERLSITKSACGRNGKQFCSTLTLNTAQANHTGFYSCKYLAVPTSKKKETESAIYIFISDTGRPFVEMYSEIPEIIHMTEGRELVIPCRVTSPNITVTLKKFPLDTLIPDGKRIIWDSRKGFIISNATYKEIGLLTCEATVNGHLYKTNYLTHRQTNTIIDVQISTPRPVKLLRGHTLVLNCTATTPLNTRVQMTWSYPDEKNKRASVRRRIDQSNSHANIFYSVLTIDKMQNKDKGLYTCRVRSGPSFKSVNTSVHIYDKAFITVKHRKQQVLETVAGKRSY

Reconstitution:

Soluble in water and most aqueous buffers. Add deionized water to the vial to fully solubilize the protein to a concentration of > 0.1 mg/ml.

Storage/Stability:

Lyophilized protein is stable over six months at -20°C to -80°C.