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Recombinant Human VEGF 189

Molecule:

VEGF

Synonyms:

Vascular endothelial growth factor A, VEGF-A, Vascular permeability factor, VPF, VEGF189

Species:

Human

Cat. No.:

CRV114B pdf (datasheet)

Quantity/Size:

5 μg

Price:

$430.00 BUY

Description:

VEGF is a member of the platelet-derived growth factor family. It is a specific mitogen for vascular endothelial cells and a strong angiogenic factor in vivo. Five different proteins are generated by diffential splicing: VEGF121, VEGF145, VEGF165, VEGF189 and VEGF206. The most abdundant form is VEGF165. Whereas VEGF121 and VEGF165 are secreted proteins, VEGF145, VEGF189 and VEGF206 are strongly cell-associated. The isoforms VEGF145, VEGF165 and VEGF189 bind to heparin with high affinity. VEGF165 is apparently a homodimer, but preparations of VEGF165 show some heterogeneity on SDS gels, depending on the secretion of different glycosylation patterns. All dimeric forms have similar biological activities but their bio-availability is very different. There is good evidence that heterodimeric molecules between the different isoforms also exist and that different cells and tissues express different VEGF isoforms. The other members of this increasing growth factor family are VEGF-B, -C, -D and -E. Another member is the Placenta growth factor PlGF.

Uniprot Name:

Vascular endothelial growth factor A

Uniprot ID:

P15692-2

Data PDF:

CRV114

Source:

E. coli

Molecular Weight:

~40 kDa (189 aa) homodimer on SDS-PAGE, non-reduced

Formulation:

Lyophilized from 50 mM Acetic Acid

Purity:

>98% by SDS-PAGE, visualized with silver stain

Biological Activity:

ED50 typically 2-10 ng/ml, determined by a cell proliferation assay using primary human umbilical vein endonthelial cells (HUVEC) and primary human dermal lymphatic endothelial cells (HDLEC).

Endotoxin Level:

< 1 EU/μg

Amino Acid Sequence:

APMAEGGGQN HHEVVKFMDV YQRSYCHPIE TLVDIFQEYP DEIEYIFKPS CVPLMRCGGC CNDEGLECVP TEESNITMQI MRIKPHQGQH IGEMSFLQHN KCECRPKKDR ARQEKKSVRG KGKGQKRKRK KSRYKSWSVP CGPCSERRKH LFVQDPQTCK CSCKNTDSRC KARQLELNER TCRCDKPRR

Reconstitution:

Centrifuge vial prior to opening. Reconstitute in water to a concentration no less than 50 µg/ml. For long term storage, we recommend adding at least 0.1% HSA or BSA.

Storage/Stability:

The lyophilized protein is stable for 1 year at -20°C to -80°C. After reconstitution, store in working aliquots at -20°C to -80°C. Avoid repeated freeze-thaw cycles.

Application:

ELISA, FS