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  • Recombinant Human TPA, >95% Active Two Chain

Recombinant Human TPA, >95% Active Two Chain

Molecule:

TPA

Synonyms:

Tissue-type plasminogen activator, t-PA, t-plasminogen activator, tPA

Species:

Human

Cat. No.:

CRT171B pdf (datasheet)

Quantity/Size:

100 μg

Price:

$670.00 BUY

Description:

Recombinant Human tPA is two-chain, activated from the single-chain form with immobilized Plasmin. It has 100% complex formation with Human PAI1.

Tissue Plasminogen Activator (tPA) is a secreted serine protease that converts the proenzyme Plasminogen to Plasmin, a fibrinolytic enzyme. tPA is synthesized as a single chain which is cleaved by Plasmin to a two chain disulfide linked protein. This enzyme plays a role in cell migration and tissue remodeling. Increased enzymatic activity causes hyperfibrinolysis, which manifests as excessive bleeding. Decreased activity leads to hypofibrinolysis, which can result in thrombosis or embolism.

Concentration:

1.0 mg/ml, lot specific

Uniprot Name:

Tissue-type plasminogen activator

Uniprot ID:

P00750

Data PDF:

CRT171B

Source:

CHO cells

Molecular Weight:

70 kDa

Formulation:

0.4 M HEPES, 0.1 M NaCl, pH 7.4

Purity:

>95% by SDS-PAGE analysis.

Biological Activity:

>1,000,000 IU/mg, lot specifc (Relative to WHO International Standard for Human Recombinant Tissue Plasminogen Activator, NIBSC 98/714)

Storage/Stability:

Store in working aliquots at -80°C. Avoid repeated freeze-thaw cycles.

Application notes:

Useful for cell differentiation, cell signaling, clotting assays, fibrinolysis, plasminogen activation, protein-protein interactions, receptor binding, thromboelastography, and thrombolysis.