Recombinant Human TNF-alpha, GMP Grade

Synonyms:

Tumor necrosis factor, Cachectin, TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNF-a

Species:

Human

Cat. No.:

CS481A-GMP pdf (datasheet)

Quantity/Size:

5 μg

Price:

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Description:

Tumor necrosis factor alpha (TNF-α) is the best-know member of the TNF-family, which can cause cell death. This protein is produced by neutrophils, activated lymphocytes, macrophages, NK cells, LAK cells, astrocytes endothelial cells, smooth muscle cells and some transformed cells. TNF-α occurs as a secreted, soluble form and as a membrane-anchored form, both of which are biologically active. The naturally-occurring form of TNF-α is glycosylated, but non-glycosylated recombinant TNF-α has comparable biological activity. The biologically active native form of TNF-α is reportedly a trimer. Human and murine TNF-α show approximately 79% homology at the amino acid level and cross-reactivity between the two species.
Manufacturing and testing of this product complies with ICH Q7 guidelines.

Entrez Symbol:

TNF

Uniprot Name:

Tumor necrosis factor

Uniprot ID:

P01375

Data PDF:

CS481-GMP

Source:

E. coli

Molecular Weight:

~17.5 kDa (158 aa) monomer

Formulation:

Lyophilized from sterile-filtered 5 mM Na2HPO4, 5 mM NaH2PO4, 10 mM NaCl, pH 7.0.

Purity:

>98% by SDS-PAGE and HPLC analyses.

Biological Activity:

ED50 < 0.05 ng/ml, by dose-dependent cytotoxicity assay using murine L929 cells

Specific Activity:

> 2.0 × 107 IU/mg in the presence of actinomycin D, which is calibrated against rHuTNF-α/TNFSF2 WHO International Standard (NIBSC code: 12/154).

Endotoxin Level:

< 0.01EU/μg as determined by LAL method.

Amino Acid Sequence:

MVRSSSRTPS DKPVAHVVAN PQAEGQLQWL NRRANALLAN GVELRDNQLV VPSEGLYLIY SQVLFKGQGC PSTHVLLTHT ISRIAVSYQT KVNLLSAIKS PCQRETPEGA EAKPWYEPIY LGGVFQLEKG DRLSAEINRP DYLDFAESGQ VYFGIIAL

Storage/Stability:

Lyophilized vial is shipped at ambient temperature. Immediately upon receipt, store unopened vial at -20°C (manual defrost freezer) or -80°C for up to 36 months. Upon reconstitution, prepare working aliquots and store at -20°C (manual defrost freezer) or -80°C. It is recommended that a carrier protein such as 0.1% HSA or BSA is added for long term storage. For short term storage reconstituted material is stable for 4-6 weeks when stored at 2-8°C. Avoid repeated freeze-thaw cycles.

Application notes:

For ex vivo use only. Not for injection or diagnostic procedures. The safety and efficacy of this product in diagnostic or other clinical uses has not been established.