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Recombinant Human TNF-alpha (aa 77-233)

Molecule:

TNF-alpha

Synonyms:

TNF-alpha, TNFA, TNFSF2, DIF

Species:

Human

Cat. No.:

CSI15659B pdf (datasheet)

Quantity/Size:

500 μg

Price:

$775.00 BUY

Description:

TNF-alpha is produced by neutrophils, CD4+ T-cells, macrophage, NK cells, LAK cells, astrocytes endothelial cells, and smooth muscle cells. TNF-alpha is cytolytic and plays an important role in immune regulation, including hemorrhagic tumor necrosis/cytotoxicity and inflammation, and in regulation of antiviral and immune proliferative and activation responses. The active form of this protein is a trimer. Recombinant human TNF-alpha contains an additional amino acid, methionine, attached at the N-terminus.

Concentration:

1 mg/mL

Uniprot Name:

Tumor necrosis factor

Uniprot ID:

P01375

Data PDF:

CSI15659

Source:

E. coli

Molecular Weight:

17.5 kDa (158 aa), amino acid sequence confirmed by MALDI-TOF

Formulation:

PBS, pH 7.4

Purity:

>95% as determined by SDS-PAGE

Biological Activity:

ED50 < 0.2 ng/mLl, measured in a cytotoxicity assay using L929 mouse fibrosarcoma cells in the presence of metabolic inhibitor actinomycin D

Endotoxin Level:

<1.0 EU/µg

Amino Acid Sequence:

MVRSSSRTPS DKPVAHVVAN PQAEGQLQWL NRRANALLAN GVELRDNQLV VPSEGLYLIY SQVLFKGQGC PSTHVLLTHT ISRIAVSYQT KVNLLSAIKS PCQRETPEGA EAKPWYEPIY LGGVFQLEKG DRLSAEINRP DYLDFAESGQ VYFGIIAL

Storage/Stability:

Product may be stored at 2-8°C for two weeks. For long term, aliquot and store at -20 °C to -80 °C. Avoid repeated freeze-thaw cycles.