Recombinant Human Tissue Plasminogen Activator

Molecule:

Plasminogen

Synonyms:

Tissue-type plasminogen activator, tPA, t-PA, t-plasminogen activator, TPA, T-PA

Species:

Human

Cat. No.:

CSI12668 pdf (datasheet)

Quantity/Size:

100 μg

Price:

$580.00 BUY

Description:

Tissue plasminogen activator (tPA) is a secreted serine protease which converts the proenzyme plasminogen to plasmin, a fibrinolytic enzyme. Plasminogen is synthesized as a single chain which is cleaved by PLAT into the two chain disulfide linked plasmin. This enzyme plays a role in cell migration and tissue remodeling. Increased enzymatic activity causes hyperfibrinolysis, which manifests as excessive bleeding; decreased activity leads to hypofibrinolysis which can result in thrombosis or embolism.

Uniprot Name:

Tissue-type plasminogen activator

Uniprot ID:

P00750

Data PDF:

CSI12667

Source:

CHO cells

Molecular Weight:

59 kDa (527 aa)

Formulation:

Lyophilized from a sterile-filtered solution containing L-arginine, phosphoric acid and Tween-80.

Purity:

> 98% as determined by SDS-PAGE and RP-HPLC analyses

Biological Activity:

580,000 IU/mg

Endotoxin Level:

< 0.1 ng/µg of TPA

Reconstitution:

Centrifuge vial prior to opening. Add sterile distilled water to the vial to fully solubilize the protein to a concentration of > 0.1 mg/ml. After complete solubilization of the protein, it can be further diluted to other aqueous solutions.

Storage/Stability:

Stored at -20°C to -80°C for up to 1 year. Upon reconstitution, store at 2-8°C for up to 1 week. For longer term, prepare aliquots and stored at -20°C to -80°C. It is recommended to add a carrier protein such as 0.1% HSA or BSA. Avoid repeat freeze-thaw cycles.