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Recombinant Human Thrombin, Active

Molecule:

Thrombin

Synonyms:

Prothrombin, Coagulation factor II, F2

Species:

Human

Cat. No.:

CS433B pdf (datasheet)

Quantity/Size:

50 ug

Price:

$1,215.00 BUY

Description:

Thrombin enzyme (Activated Factor IIa) is an important clotting promoter that controls the transformation of soluble fibrinogen to insoluble active fibrin strands. Thrombin is a coagulation protein and a serine protease (EC 3.4.21.5) that catalyzes many coagulation-related reactions. Thrombin triggers factor-XI, factor-V, Factor-XIII and factor-VIII. Thrombin endorses platelet activation, using activation of protease-activated receptors on the platelet. As a result of its high proteolytic specificity, thrombin has become an important biochemical protein. The thrombin cleavage site (Leu-Val-Pro-Arg-Gly-Ser) is widely used in linker regions of recombinant fusion protein constructs. After the purification of the fusion protein, thrombin is used to cleave between the Arginine and Glycine residues of the cleavage site, efficiently removing the purification tag from the protein of interest with a high degree of specificity.

Entrez Symbol:

F2

Uniprot Name:

Prothrombin

Uniprot ID:

P00734

EC number:

EC 3.4.21.5

Data PDF:

CS433

Source:

HEK293 cells

Molecular Weight:

36 kDa

Formulation:

Sterile filtered 20mM MES, pH 6.0 containing 500mM choline chloride

Purity:

> 95% by SDS-PAGE

Biological Activity:

5396 NIH Units/mg.
The activity was determined in NIH units by comparing to Sigma’s human plasma thrombin. Protein concentration was measured using E(0.1%)@280nm = 1.83.

Storage/Stability:

Store at -20°C to -80°C. Avoid repeated freeze-thaw cycles.

Application notes:

Thrombin is a valuable biochemical tool used to cleave the thrombin cleavage site (LeuVal-Pro-Arg-*-Gly-Ser) that is commonly included in linker regions of recombinant fusion protein constructs.