Synonyms:
Receptor tyrosine-protein kinase erbB-4, Proto-oncogene-like protein c-ErbB-4, Tyrosine kinase-type cell surface receptor HER4 p180erbB4
Cat. No.:
CRH466A-His
pdf (datasheet)
Description:
ERBB4 is a single-pass type I membrane protein with multiple cysteine rich domains, a
transmembrane domain, a tyrosine kinase domain, a phosphotidylinositol-3 kinase
binding site and a PDZ domain binding motif. ERBB4 is expressed at highest levels in
brain, heart, kidney, in addition to skeletal muscle, parathyroid, cerebellum, pituitary,
spleen, testis and breast. And lower levels in thymus, lung, salivary gland, and pancreas.
It specifically binds to and is activated by neuregulins, NRG-2, NRG-3, heparin-binding
EGF-like growth factor, betacellulin and NTAK. ERBB4 also can be activated by other
factors and induces a variety of cellular responses including mitogenesis and
differentiation. ERBB4 regulates development of the heart, the central nervous system
and the mammary gland, gene transcription, cell proliferation, differentiation, migration
and apoptosis. It is required for normal cardiac muscle differentiation during embryonic
development, and for postnatal cardiomyocyte proliferation. ERBB4 also play a role on
the normal development of the embryonic central nervous system, especially for normal
neural crest cell migration and normal axon guidance.
Uniprot Name:
Receptor tyrosine-protein kinase erbB-4
Genbank Accession No:
NP_005226.1
Molecular Weight:
The recombinant human ERBB4 comprises 635 amino acids and has a predicted molecular mass of 71.1 kDa. The apparent Mr of the protein is ~92 kDa in SDS-PAGE under reducing conditions.
Formulation:
Lyophilized from sterile PBS, pH 7.4.
Normally 5% - 8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Purity:
> 95 % as determined by SDS-PAGE
Biological Activity:
In a functional ELISA, immobilized human ERBB4-His (1-649) at 10 μg/ml (100 μl/well) can bind biotinylated human NRG1, The EC50 of biotinylated human NRG1 is 0.49-1.13 μg/ml.
Endotoxin Level:
< 1.0 EU per μg of the protein as determined by the LAL method
Reconstitution:
Centrifuge vial prior to opening. Add sterile distilled water to a concentration of 0.1 mg/mL and gently pipette the solution up and down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution.
Storage/Stability:
Stable for up to 1 year from date of receipt at -20°C to -80°C After reconstitution, store working aliquots at -20°C to -80°C. Avoid repeated freeze-thaw cycles.
