CCL5

RANTES, SCYA5, SISd, TCP228, SIS-delta, T-cell specific protein p288, Regulated upon activation, normally T-expressed, and presumably secreted, Small inducible cytokine A5

Human

CRR000A pdf (datasheet)

5 μg

$190.00 BUY

Recombinant Human RANTES/CCL5 is a single non-glycosylated polypeptide chain containing 68 amino acids.

Background: RANTES/CCL5 (acronym for Regulated upon Activation, Normal T cell Expressed and presumably Secreted), was initially discovered by subtractive hybridization as a transcript expressed in T cells but not B cells. Eosinophil chemotactic activities released by thrombin stimulated human platelets have also been purified and found to be identical to RANTES. Besides T cells and platelets, RANTES has been reported to be produced by renal tubular epithelium, synovial fibroblasts and selected tumor cells.

CRR000

E. coli

~7.8 kDa

Lyophilized from a 0.2 μm filtered concentrated solution in 20mM PB, pH 7.4, 100 mM NaCl.

>98 % by SDS-PAGE and HPLC analyses.

Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human peripheral blood monocytes is in a concentration range of 1.0-10 ng/ml.

Less than 1EU/μg of recombinant human RANTES/CCL5 as determined by LAL method.

SPYSSDTTPC CFAYIARPLP RAHIKEYFYT SGKCSNPAVV FVTRKNRQVC ANPEKKWVRE YINSLEMS

Centrifuge vial prior to opening. Add sterile distilled water or aqueous buffer to a concentration of 0.1-1.0 mg/ml. Further dilutions should be made in appropriate buffered solutions.

This lyophilized preparation is stable at 2-8°C, but should be kept desiccated at -20°C for long term storage. Upon reconstitution, the preparation is stable for up to one week at 2-8°C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20°C to -80°C. Avoid repeated freeze/thaw cycles.