Recombinant Human RAF1 (EE) GST tag, Active

Synonyms:

RAF proto-oncogene serine/threonine-protein kinase, Proto-oncogene c-RAF, Raf-1

Species:

Human

Cat. No.:

CRR117C pdf (datasheet)

Quantity/Size:

100 μg

Price:

$2,590.00 BUY

Description:

RAF1 is a MAP kinase kinase kinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. The activated RAF1 can phosphorylate and activate the dual specificity protein kinases MEK1 and MEK2, which in turn phosphorylate to activate the serine/threonine specific protein kinases ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration.

Active rhRAF1 (Y340E, Y341E, 306-end) was expressed by baculovirus in Sf9 cells with an N-terminal GST tag.  RAF1 (EE) is 100-fold more active vs. wild type.

Concentration:

0.10 mg/ml

Entrez Symbol:

RAF1

Uniprot Name:

RAF proto-oncogene serine/threonine-protein kinase

Uniprot ID:

P04049

Data PDF:

CRR117

Source:

Sf9 insect cells

Molecular Weight:

63 kDa

Formulation:

50mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25% glycerol

Purity:

>75% by SDS-PAGE

Specific Activity:

3,000-5,000 nmol/min/mg, lot specific

Storage/Stability:

Store at -80°C for up to 1 year.