Recombinant Human Protein Kinase C delta, Active (GST Tag)

Synonyms:

Protein kinase C delta type, Tyrosine-protein kinase PRKCD, nPKC-delta

Species:

Human

Cat. No.:

CRP114B pdf (datasheet)

Quantity/Size:

10 μg

Price:

$550.00 BUY

Description:

Protein kinase C delta (PKCδ) is a member of the protein kinase C (PKC) family of serine-threonine kinases. It shows strict dependence on the presence of phospholipids, but shows no activation by Ca2+. Phosphatidylinositol is the most potent activator of PKC delta. Northern blot analysis indicated that PKC delta is widely distributed in almost all the tissues and is a major isoform of PKC expressed in hemopoietic cells. PKC delta is involved in fundamental cellular functions regulated by diacylglycerols and mimicked by phorbol esters.

Concentration:

0.1 mg/ml

Uniprot Name:

Protein kinase C delta type

Uniprot ID:

Q05655

Data PDF:

CRP114

Source:

Sf9 insect cells

Molecular Weight:

~104 kDa (79 kDa protein kinase + ~25 kDa GST tag)

Tag:

GST

Formulation:

Recombinant protein stored in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.25 mM DTT, 0.1 mM EDTA, 0.1 mM PMSF, 25% Glycerol.

Purity:

>90% determined by densitometry.

Specific Activity:

250 nmol/min/mg per activity assay (protocol included)

Storage/Stability:

Stable for 1 year at -80°C from date of receipt. Upon initial thaw, briefly centrifuge the vial to consolidate product, prepare aliquots (>20 ml) and store -80°C . Avoid repeated freeze-thaw cycles.