Recombinant Human PDGFR-beta, C-terminal fragment contains amino acids R561-L1106 (the cytoplasmic domain) with an N-terminal GST tag with a FactorXa cleavage site.

Amino Acid Sequence: 

MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD LIEGRGIPGN SRYEIRWKVI ESVSSDGHEY IYVDPMQLPY DSTWELPRDQ LVLGRTLGSG AFGQVVEATA HGLSHSQATM KVAVKMLKST ARSSEKQALM SELKIMSHLG PHLNVVNLLG ACTKGGPIYI ITEYCRYGDL VDYLHRNKHT FLQHHSDKRR PPSAELYSNA LPVGLPLPSH VSLTGESDGG YMDMSKDESV DYVPMLDMKG DVKYADIESS NYMAPYDNYV PSAPERTCRA TLINESPVLS YMDLVGFSYQ VANGMEFLAS KNCVHRDLAA RNVLICEGKL VKICDFGLAR DIMRDSNYIS KGSTFLPLKW MAPESIFNSL YTTLSDVWSF GILLWEIFTL GGTPYPELPM NEQFYNAIKR GYRMAQPAHA SDEIYEIMQK CWEEKFEIRP PFSQLVLLLE RLLGEGYKKK YQQVDEEFLR SDHPAILRSQ ARLPGFHGLR SPLDTSSVLY TAVQPNEGDN DYIIPLPDPK PEVADEGPLE GSPSLASSTL NEVNTSSTIS CDSPLEPQDE PEPEPQLELQ VEPEPELEQL PDSGCPVPRA EAEDSFL

1-218 (underlined): GST     Italic: Factor Xa cleavage site      Bold: PDGFR-beta fragment

Background: PDGFR-beta is a cell surface tyrosine kinase receptor for members of the platelet-derived growth factor family. These growth factors are mitogens for cells of mesenchymal origin. The PDGF isoforms AA, BB, AB, CC, and DD are dimeric molecules. They bind two receptor molecules simultaneously and dimerize them upon binding. PDGFR-alpha binds the A, B, and C chains of PDGF with high affinity, whereas PDGFR-beta binds the B and D chain with high affinity.