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Recombinant Human PAK4/p21, Active

Synonyms:

Serine/threonine-protein kinase PAK 4, p21-activated kinase 4

Species:

Human

Cat. No.:

CRP105A pdf (datasheet)

Quantity/Size:

5 μg

Price:

$350.00 BUY

Description:

PAK4 is a recently identified member of the p21-activated kinases (PAKs) which have been implicated in the regulation of cell morphology, motility and transformation. These serine/threonine kinases are activated by and are effectors of small GTPases, cdc 42 and Rac. PAK4 belongs to the Group II PAKs which also includes PAK5 and PAK6 while Group I PAKs comprise of PAK1, PAK2 and PAK3. PAK4 differs from other members of the PAK family both in sequence and function. PAK4 has been shown to regulate cell morphology and cytoskeletal organization in mammalian cells.

Concentration:

0.1 mg/ml

Entrez Symbol:

PAK4

Uniprot Name:

Serine/threonine-protein kinase PAK 4

Uniprot ID:

O96013

Data PDF:

CRP105

Source:

Insect cells

Molecular Weight:

approximately 90 kDa

Tag:

N-terminal GST

Formulation:

50 mM Tris-HCl, 150 mM NaCl, 10 mM glutathoine, 0.25 mM DTT, 0.1 mM EDTA, 0.1 mM PMSF, 25% Glycerol, pH 7.5

Purity:

> 90% by SDS-PAGE (Coomassie)

Specific Activity:

Kinase Specific Activity >50 nmol/min/mg: nmol phosphate incorporated into the PKB-sub peptide (CKRPRAASFAE) per minute per mg protein at 30ºC for 15 minutes using a final concentration of 50 µM ATP (0.83 µCi/assay).

Storage/Stability:

Store in working aliquots at -80°C. Avoid repeated freeze-thaw cycles.