Synonyms:
Neurotrophin-4, Neurotrophin 5, NT-5, Neurotrophic Factor 4
Description:
Neurotrophin-4 (NT-4) is an important member of the nerve growth factor (NGF) family of proteins. Neurotrophins undergo paracrine and autocrine signaling to control neuronal survival, neuronal differentiation, and dendrite outgrowth. NT-4 is expressed ubiquitously and signals through the TrkB receptor tryrosine kinase.
Uniprot Name:
Neurotrophin-4
Molecular Weight:
14.1/28.1 kDa, (131/262 aa) noncovalant homodimer
Formulation:
Lyophilized from a sterile-filtered solution containing 0.1% Trifluoroacetic Acid
Purity:
≥95% by reducing and non-reducing SDS-PAGE
Biological Activity:
ED50 < 20 ng/ml by dose-dependent proliferation of a neuroblastoma cell line stably expressing TrkB (BR6).
Endotoxin Level:
≤ 0.1 EU/µg by kinetic LAL analysis
Amino Acid Sequence:
MGVSETAPAS RRGELAVCDA VSGWVTDRRT AVDLRGREVE VLGEVPAAGG SPLRQYFFET RCKADNAEEG GPGAGGGGCR GVDRRHWVSE CKAKQSYVRA LTADAQGRVG WRWIRIDTAC VCTLLSRTGR A
Storage/Stability:
Store as supplied at -20°C to -80°C for up to 1 year. Upon reconstitution, the preparation is stable for up to one month at 2-8°C. For long term storage reconstitute in working aliquots containing 0.1% BSA and store at -80°C. Avoid repeated freeze-thaw cycles.
