Recombinant Human/Mouse Neurotrophin 3

Molecule:

NTF3

Synonyms:

Neurotrophic factor, Nerve growth factor-2, NGF-2, HDNF, NT3

Species:

Mouse | Human

Cat. No.:

CRN500B pdf (datasheet)

Quantity/Size:

10 μg

Price:

$230.00 BUY

Description:

Neurotrophin-3 (NTF3) is an important member of the nerve growth factor (NGF) family of proteins behind BDNF and NGF. It is thought to promote the survival or differentiation of existing and new neurons in the central nervous system and synapses. These functions are thought to be communicated by TrkC, a receptor tryrosine kinase thought induce NTF3-specific signaling. In addition, NTF3 is thought to also bind TrkB and low affinity nerve growth factor receptor (LNGFR).

Recombinant Human and mouse NTF3 have 100% sequence homology.

Entrez Symbol:

NTF3

Uniprot Name:

Neurotrophin-3

Uniprot ID:

P20783 human, P20181 mouse

Data PDF:

CRN500

Source:

E. coli

Molecular Weight:

Dimer (noncovalently linked), 13.8/27.5 kDa (120/240 aa)

Formulation:

Lyophilized from a sterile filtered solution containing 0.1% Trifluoroacetic Acid (TFA).

Purity:

≥95% by reducing and non-reducing SDS-PAGE

Biological Activity:

ED50 < 20 ng/ml by dose-dependent  proliferation of a neuroblastoma cell line stably expressing TrkB (BR6).

Endotoxin Level:

≤ 1 EU/µg by kinetic LAL

Amino Acid Sequence:

MYAEHKSHRG EYSVCDSESL WVTDKSSAID IRGHQVTVLG EIKTGNSPVK QYFYETRCKE ARPVKNGCRG IDDKHWNSQC KTSQTYVRAL TSENNKLVGW RWIRIDTSCV CALSRKIGRT

Reconstitution:

Centrifuge vial prior to opening. Add sterile distilled water to a concentration of 0.1 mg/mL and gently pipette the solution up and down the sides of the vial. DO NOT VORTEX. Allow several minutes for reconstitution.

Storage/Stability:

Upon receipt, store as supplied at -20°C to -80°C for up to one year. Upon reconstitution, the preparation is stable for up to one month at 2-8°C. For long term storage reconstitute in working aliquots containing 0.1% BSA and store at -20°C to -80°C for up to 3 months. Avoid repeated freeze-thaw cycles