NRG1

Pro-neuregulin-1, membrane-bound isoform, Pro-NRG1, Neuregulin-1

Human

CRN005A-GMP pdf (datasheet)

5 μg

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Neuregulin 1 belongs to a family of structurally related polypeptide growth factors and is produced in numerous isoforms by alternative splicing, which allows it to perform a wide variety of functions. These isoforms include heregulins (HRGs), glial growth factors (GGFs) and sensory and motor neuron-derived factor (SMDF). They all have the Ig and EGF-like domain, and can bind to ErbB3 and ErbB4 receptor tyrosin kinases. This binding induces ErbB3 and ErbB4 heterodimerization with ErbB2, stimulating intrinsic kinase activity, which leads to tyrosine phosphorylation. NRG1 isoforms have functions of inducing the growth and differentiation of epithelial, neuronal, glial, and other types of cells.
Manufacturing and testing of this product complies with ICH Q7 guidelines.

NRG1

Pro-neuregulin-1, membrane-bound isoform

Q02297

CRN005-GMP

E. coli

~7.0 kDa (61 aa) monomer

Lyophilized from sterile-filtered PBS, pH 7.4

> 98.0% by HPLC and SDS-PAGE

ED50 < 5.0 ng/ml, determined by dose-dependent cell proliferation assay using serum free human MCF-7 cells.

> 2.0 × 10e5 U/mg

< 0.01 EU/μg protein as determined by LAL method

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Lyophilized vial is shipped at ambient temperature. Immediately upon receipt, store unopened vial at -20°C (manual defrost freezer) or -80°C for up to 36 months. Upon reconstitution, prepare working aliquots and store at -20°C (manual defrost freezer) or -80°C. It is recommended that a carrier protein such as 0.1% HSA or BSA is added for long term storage. For short term storage reconstituted material is stable for 4-6 weeks when stored at 2-8°C. Avoid repeated freeze-thaw cycles.

For ex vivo use only. Not for injection or diagnostic procedures. The safety and efficacy of this product in diagnostic or other clinical uses has not been established.