Tumor necrosis factor ligand superfamily member 13, A proliferation-inducing ligand, APRIL, TNF- and APOL-related leukocyte expressed ligand 2, TALL-2, TNF-related death ligand 1 , TRDL-1, CD256, Adiponectin, Adipocyte complement-related 30 kDa protein, ACRP30

Human

CRH025 pdf (datasheet)

10 μg

$500.00 BUY

APRIL is a cytokine that belongs to the TNF superfamily and binds to TACI and BCMA. It is implicated in the regulation of tumor cell growth, is involved in monocyte/macrophagemediated immunological processes and functions as an important survival factor for plasmablasts and bone marrow plasma cells. Multimeric APRIL is a high activity construct in which two trimeric APRIL ligands are artificially linked via the collagen domain of ACRP30. This construct very effectively stimulates proliferation B cell. A basic amino acid sequence (QKQKKQ) close to the N-terminus of APRIL is required for binding to negatively charged sulfated glycosaminoglycan side chains of proteoglycans. Proteoglycans mediate binding of APRIL to tumor cells as well as primary lymphoid cells. Human APRIL aa 88-233 is fused at the N-terminus to human collagen domain of ACRP30 (aa16-108) and a FLAG tag.

0.1 mg/ml after reconstitution

Human APRIL: O75888
Human ACRP30 (adiponectin): Q15848

CRH025

HEK293 cells

~34 kDa by SDS-PAGE

Lyophilized from PBS

≥95% (SDS-PAGE)

Stimulates B cell proliferation

<0.02 EU/μg purified protein as determined by LAL test (Lonza)

The extracellular domain of human APRIL (aa 88-233) is fused at the N-terminus to human ACRP30 headless (aa 16-108) and a FLAG-tag.

Reconstitute with 100μl sterile water.

Store at -20°C to -80°C for up to 1 year.