MIF

GIF, GLIF, MMIF, glycosylation-inhibiting factor, phenylpyruvate tautomerase

Human

CSI20147B pdf (datasheet)

50 μg

$275.00 BUY

Recombinant Human MIF is a single non-glycosylated polypeptide chain containing 115 amino acids.

Background: Migration Inhibitory Factor (MIF) consists of two alpha-helices and six beta-strands, four of which form a beta-sheet. The two remaining beta-strands interact with other MIF molecules, creating a trimer. Structure-function studies suggest MIF is bifunctional with segregated topology. The N- and C-termini mediate enzyme activity (in theory). Phenylpyruvate tautomerase activity (enolto-keto) has been demonstrated and is dependent upon Pro at position 1. Amino acids 50-65 (a.a.) have also been suggested to contain thiol-protein oxidoreductase activity. MIF has proinflammatory cytokine activity centered around (a.a.) 49 - 65. On fibroblasts, MIF induces, IL-1, IL-8 and MMP expression; on macrophages, MIF stimulates NO production and TNF-alpha release folllowing IFN-gamma activation. MIF apparently acts through CD74 and CD44, likely in some form of trimeric interaction. Human MIF is active on mouse cells. Human MIF is 90%, 94%, 95%, and 90% aa identical to mouse, bovine, porcine and rat MIF, respectively.

CSI20147

E. coli

~12.5 kDa

Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.

>97 % by SDS-PAGE and HPLC analyses.

Fully biologically active when compared to standard. The specific activity is determined by binding rhCD74 in a functional ELISA.

Less than 1 EU/μg of recombinant human MIF as determined by LAL method.

MPMFIVNTNV PRASVPDGFL SELTQQLAQA TGKPPQYIAV HVVPDQLMAF GGSSEPCALC SLHSIGKIGG AQNRSYSKLL CGLLAERLRI SPDRVYINYY DMNAANVGWN NSTFA

Centrifuge vial prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at <-20°C. Further dilutions should be made in appropriate buffered solutions.

This lyophilized preparation is stable at 2-8°C, but should be kept at -20°C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8°C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20°C to -70°C. Avoid repeated freeze/thaw cycles.